Microsart® Calibration Reagent Mycoplasma pneumoniae

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific Mycoplasma species per vial.

Catalog No.: 
SMB95-2024
Piece
1

*Custom/bulk order quotes are provided within 24 hours of request.

Microsart® Calibration Reagents provide isolated genomic DNA of a specific Mycoplasma species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a special liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

Applications

  • Application
    8799047909984
  • Application Area
    • Schnelltest
    • 快速检测
    • Rapid Testing
    • Teste rápido
    • Test rapide
    • Pruebas Rápidas

Biological Information

  • Species
    8799085691488

Components

  • Additional Required Components
    • qPCR 循环仪
    • qPCR cycler
    • PCR 反应管
    • PCR reaction tubes
    • 用于 1.5 mL PCR 管的离心机
    • centrifuge for 1.5 ml PCR tubes
    • pipettes
    • 移液器
    • PCR 试剂盒
    • PCR kit

Dimensions

  • Filter Diameter
    N/A

General Specifications

  • Culture Media
    8801139851872
  • Detection Method
    • PCR
    • PCR
    • PCR
    • PCR
    • PCR
    • PCR
    • PCR
    • PCR
    • PCR
  • Membrane Color | Grid Color
    8801139786336

Materials of Construction

  • Filter Material
    8801172128352

Physicochemical Information

  • Pore Size
    N/A

Product Information

  • Brand
    8798948917856
  • Pack Size
    N/A
  • Type
    Reagents
  • What you receive
    • 1 vial with 10^8 genome copies lyophilized
    • 3 vials buffer
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